Peptide Phage Display
Structured Peptide Libraries
Dyax has created a number of small, disulfide-constrained cyclic peptide libraries.
- The peptides within the cyclic structure range in size from six to twelve amino acids.
- Amino acids both within and outside the cyclic structure are varied.
- The number of distinct peptide structures in each library exceeds 1 billion.
- In total, these libraries contain about 10 billion distinct peptides.
Peptides resulting from library screening are chemically synthesized and may be chemically modified. These libraries are preferred for in vivo imaging and affinity separations applications. They are validated against many different targets.
Substrate Phage Peptide Library
We have designed and built a substrate phage peptide library. This library was built by varying 13 positions of a linear peptide of which each position 19 amino acids are allowed (Cysteine is excluded). This creates a library of approximately 100 million peptides.
Each displayed peptide starts with a constant streptavidin binding domain. Displayed phage are bound to immobilized streptavidin. These are then treated with the protease of interest. The phage that are released are grown and sequenced. The substrate sequence can be inferred from the collection of sequences.
Dyax has used our peptide substrate libraries to identify novel peptides that are better substrates for proteases when compared to their native substrates. In some cases, Dyax has identified substrates to novel orphan proteases, where their native substrate is not known.
